Semiconductor Physics, Quantum Electronics & Optoelectronics. 2009. V. 12, N 1. P. 091-097.


Detection of biomolecules using optoelectronic biosensor based on localized surface plasmon resonance. Nanoimprint lithography approach
V. Chegel1, B. Lucas2, J. Guo2, A. Lopatynskyi1, O. Lopatynska3, L. Poperenko3

1V. Lashkaryov Institute of Semiconductor Physics, National Academy of Sciences of Ukraine, 41, prospect Nauky, 03028 Kyiv, Ukraine, Phone: +38 (044) 525-56-26, e-mail: vche111@yahoo.com, lop2000@ukr.net
2The University of Michigan, 1301 Beal Avenue, Ann Arbor, MI 48109, USA, E-mail: guo@umich.edu
3Taras Shevchenko Kyiv National University, build. 1, 2, prospect Glushkova, 03022 Kyiv, Ukraine, Phone: +38 (044) 526-22-96, e-mail: olga_lopatynska@ukr.net, plv@univ.kiev.ua

Abstract. In this work, we use a nanoimprint lithography fabrication technology to create uniformly-oriented and homogenous noble metal nanoparticle arrays with well- controlled size, shape and spacing, which can be the basis platform for the development of Localized Surface Plasmon Resonance (LSPR) sensors for biomolecular detection. Using this approach, we demonstrate proof-of-principle of an optical biosensor to quantify biomolecular interactions in a real-time mode using a UV-visible spectrophotometer. The sensor shows concentration-dependent kinetics of surface adsorption or binding of biomolecules and a capability to monitor antigen-antibody specific reactions, which is demonstrated by the reaction between bovine serum albumin (BSA) and anti-BSA immunoglobulin.

Keywords: localized surface plasmon resonance, nanoimprint lithography, biomolecular detection.

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