Semiconductor Physics, Quantum Electronics & Optoelectronics, 20 (1), P. 096-104 (2017).
DOI: https://doi.org/10.15407/spqeo20.01.096
Correlation optical microscopy of anisotropic biological layers
Chernivtsi National University,
2, Kotsyubinsky str., 58012 Chernivtsi, Ukraine;
E-mail: a.dubolazov@chnu.edu.ua
Abstract. The theoretical background of an azimuthally stable method for Jones-matrix mapping histological sections of biopsy taken from myocardium tissue on the basis of spatial frequency selection of mechanisms responsible for linear and circular birefringence is presented. The diagnostic application of a new correlation parameter – complex degree of mutual anisotropy – has been analytically substantiated. The method for measuring coordinate distributions of complex degree of mutual anisotropy with further spatial filtration of their high- and low-frequency components has been developed. The interrelations of these distributions with parameters of linear and circular birefringence in myocardium tissue histological sections have been found. The comparative results of measuring the coordinate distributions inherent to complex degree of mutual anisotropy related with fibrillar networks of myosin fibrils typical for myocardium tissue in different necrotic states, when death is caused by coronary heart disease and acute coronary insufficiency, have been presented. The values and ranges of changes in the statistical (moments of the 1st to 4th order) parameters describing the complex degree of mutual anisotropy coordinate distributions have been studied. The objective criteria for differentiation of causes leading to death have been determined.
Keywords: polarization, Fourier optics and signal processing, imaging systems, medical and biological imaging.
A.G. Ushenko, A.V. Dubolazov, Yu.A. Ushenko, M.Yu. Sakhnovskiy, O.V. Olar, A.V. Motrich, P.O. Angelsky
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