https://doi.org/10.15407/spqeo5.03.307
Semiconductor Physics, Quantum
Electronics & Optoelectronics. 2002. V. 5, N 3. P. 307-311.
PACS: 42.40.K
Holographic interferometry as a method to study conformational changes in macromolecules
1) Institute of Physics, NAS of Ukraine,
46 prospect Nauky, 03650 Kyiv-39, Ukraine;
2) T.Shevchenko University, 64 Volodymyrs'ka vul., 04070 Kyiv, Ukraine;
3) UKMA University, 2 Scovorody vul., 04070, Kiev, Ukraine
Phone: 38(044) 570 7823. E-mail: ybar@mail.kar.net
Abstract.
This paper concerns with our measurements of reaction center (RC) volume
changes in the course of photoactivation using the method of holographic
interferometry. As a principal value, we studied changes of a solution
refraction index (Dn)
influenced by the sample volume expansion due to temperature changes (DnT),
light absorption (Dnabs)
and changes in the volume of photoactive molecules (DnV).
Using the scheme of temperature compensation we could reduce the task
to the case DnT
<<Dnabs,
DnV.
At RC concentrations close to 1015
cm-3, our experimental setup
enabled to register the shift of an interference pattern up to 0.1 of
the bandwidth. Our estimations for the case of a protein solution yields
in relative changes in the molecular volume within 10-3...10-2.
Keywords: photoinduced
change, electron transfer, holographic interferometer, photo-thermoplastical
registering media, refraction index.
Paper received 08.04.02; accepted for publication
10.12.02.
Download full text in PDF [PDF 298K]
This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License.