Semiconductor Physics, Quantum Electronics & Optoelectronics. 2001. V. 4, N 4. P. 343-346.

L.I.Berezhinsky, V.I.Chegel’, Yu.M.Shirshov
Institute of Semiconductor Physics, NAS Ukraine, 45 Prospect Nauki, Kyiv, 03028, Ukraine
Phone: (38044) 265 18 27; Fax: (38044) 265 83 42; E-mail: che@isp.kiev.ua

G.I.Dovbeshko
Insitute of Physics, NAS of Ukraine, 46 Prospect Nauki, 03028 Kyiv, Ukraine

O.V. Melnichuk
Mykola Gogol State Pedagogic University, 2 Kropyv’yans’kogo St., Nizhyn, 16600, Ukraine

Abstract. A possibility to use surface plasmon resonance (SPR) in the investigations of the microwave radiation influence upon proteins in the process of their adsorption on the gold surface and their interaction with other proteins. For the first time, this method is applied to experimental study of physical parameters of the biological molecules under the action of the weak (from 1 to 10 mW/ cm²) electromagnetic fields in real-time regime. The changes in the process of adsorption of protein on the gold at different stages of the absorption as well as the changes in the reaction of antigen-antibody were introduced under the action of microwave field. More dense layers of protein was obtained in the microwave fields resulting in the decreased contribution of non-specific and specific interactions between proteins.

Keywords: surface plasmon resonance, adsorbed layer, protein, antigene-antibody, microwave field, polarization of medium, dielectric permeability, gold film.

Paper received 13.07.01; revised manuscript received 01.12.01; accepted for publication 12.12.01.

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