Semiconductor Physics, Quantum Electronics & Optoelectronics, 20 (2), P. 159-167 (2017).

Surface enhanced imaging and IR spectroscopy of the biological cells on the nanostructured gold film
G.I. Dovbeshko1, O.P. Gnatyuk1, S.O. Karakhim2, T.P. Doroshenko3, V.R. Romanyuk3

1Institute of Physics, NAS of Ukraine, 46, prospect Nauky, 03680 Kyiv, Ukraine, Phone: +38 (044) 525-98-51, fax: +38 (044)525-15-89, e-mail:
2Palladin Institute of Biochemistry, NAS of Ukraine, 9, Leontovycha str., 01601 Kyiv, Ukraine
3V. Lashkaryov Institute of Semiconductor Physics, NAS of Ukraine, 41, prospect Nauky, 03680 Kyiv, Ukraine

Abstract. New approach for optical imaging, structural study and cell cultivation based on the effect of the enhancement of optical signals from biomolecules and biological cells near nanostructured rough gold surface is proposed. The surface enhanced IR absorption (SEIRA) spectroscopy and confocal microscopy experiments were made using the culture of SPEV (porcine embryonic kidney epithelium transplantable line) and fibroblast cells, cultivated and/or adsorbed on the gold substrate. The SEIRA spectra registered from monolayer of the SPEV cells cultivated on the rough gold showed a low frequency shift of about 2 to 7 cm 1 for the most characteristic IR vibrations, compared with those adsorbed from suspension on the same substrate. An enhancement factor of 15…30 was obtained for different molecular vibrations. The confocal microscopy contrast images of the SPEV cells on rough gold substrate were obtained in laser fluorescence mode. This approach opens new possibilities for visualization of the living cells in vivo without staining. The fluorescence of the rough gold surfaces and effects responsible for our findings have been discussed.

Keywords: SPEV cells, nanostructured rough gold substrate, SEIRA spectroscopy, laser scanning confocal microscopy, fluorescence imaging.

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